Microsatellites, also referred to Simple Sequence Repeats (SSRs), are short sequences of DNA consisting of 1-6 base pairs that are present across the genome with high variability in repeat units, making them valuable tools for genetic research including genetic mapping, forensic analysis, population genetics, and marker-assisted selection in breeding programs due to their high mutation rate, abundance, and widespread distribution in both coding and non-coding regions.

Using computational tools to analyze genomic data, researchers can efficiently identify microsatellites in tropical and sub-tropical fruit crops without extensive laboratory work, enabling detailed mapping of SSRs and further studies on genetic diversity, evolutionary biology, and molecular markers for crop improvement and conservation efforts in various fruit species.